Moffat et BMS-754807 order al. (2007) have shown that liver miRNAs in both mouse and rat respond to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) treatment, a potent AHR agonist. However, the changes in expression levels for most of the miRNAs were modest and could not be confirmed by real-time RT-PCR, suggesting that hepatic miRNAs may play only a minimal role in AHR-mediated transcriptional hepatic response. Lastly, the gene expression responses in the liver and the lungs

of the BaP exposed mice were significantly different, with lungs exhibiting a pronounced immunosuppressive expression profile, in addition to xenobiotic metabolism, p53 signalling and oxidative stress. In conclusion, we have demonstrated a strong response in mRNA and miRNA in the lungs of

mice exposed to BaP by oral gavage. The pulmonary profiles of both gene and miRNA expressions resemble those of certain Galunisertib chemical structure types of lymphomas. The present study details the molecular mechanisms underlying BaP-induced immunotoxicity in lungs and its potential implications. Further research is needed to determine whether these molecular signatures can be used as markers for screening other environmental immunotoxicants, or if the miRNA expression profiles provide a better biomarker of immunotoxicity. The present study also highlights the importance of studying non-target organ toxicity in understanding the overall effects of a widespread chemical like BaP. There are no conflicts of interest to disclose. We thank Kelly Jackson for animal experimental design and sample collection, Lynn Berndt-Weis and Julie Buick for liver mRNA microarray data, and Karen

Leingardner for clinical chemistry. We also thank Christine Lemieux and David Lefebvre for helpful comments on the manuscript. “
“Hydroquinone (HQ) and benzene play an important role in both indoor and outdoor pollution as both are present Adenosine in high concentrations in cigarette smoke, where HQ is the most pro-oxidant compound (McGregor, 2007) and benzene is an environmental pollutant released from adulterated fuel. In addition, HQ is endogenously produced during benzene biotransformation, mainly in the lungs, liver and bone marrow, and it is also responsible for the myelotoxicity and immunosuppression detected during benzene toxicity (McGregor, 2007, Snyder, 2002 and Snyder, 2004). Cells and tissues present in the respiratory system are easy targets of the toxic actions of pollutants and pathogens dispersed in the atmosphere. In this context, a pool of active resident cells is necessary to provide a protective environment against inhaled microbes and to maintain host defence effectiveness (Soehnlein and Lindbom, 2010). The functional pool of alveolar macrophages (AMs) represents 90% of haematopoietic cells in the alveolar space and is responsible for eliminating invading agents, such as particles and microorganisms, by phagocytosis and killing activities (Geiser, 2002, Gordon and Read, 2002 and Laskin et al., 2001).