Methods The cultured A375 human melanoma cells were randomly assigned to control and tacrolimus treatment groups (10, 10(2), 10(3) and 10(4) nmol/L). The cell proliferation was measured with Cell Counting Kit-8 assays. Melanin content was measured with NaOH method. Transwell migration assay was used to measure cell migration. The expression of c-KIT mRNA and protein were measured with real-time
fluorescence quantitative polymerase chain reaction and immunohistochemistry respectively. Results The cell proliferation of the 10(3) and 10(4) nmol/L tacrolimus groups were significantly lower (0.666 +/- 0.062 and 0.496 +/- 0.038) as compared with the control (0.841 +/- 0.110, P smaller than 0.05). The mean melanin content in all groups treated with different concentration of tacrolimus (10, 10(2), 10(3), 10(4) nmol/L) increased compared with the control group (P smaller than 0.05). Compound C 2HCl Dose-dependent increase in cell migration were seen in all tacrolimus-treated groups (P smaller
than 0.01). The expression of c-KIT mRNA level in A375 cells exposed to tacrolimus (103 and 104 nmol/L) had significantly increased by 3.03-fold and 3.19-fold respectively compared with the control (P smaller than 0.05). Conclusions Although tacrolimus had no effects on cell proliferation on A375 human melanoma cells, it could increase MK-0518 the melanin content and cell migration. The expression of c-KIT mRNA and protein increased dose-dependently in tacrolimus-treated groups as compared with the control. Our study demonstrated that tacrolimus could enhance the melanogenesis this website and cell migration on A375 cells.”
“Purpose: To compare the extent of tumor motion between 4-dimensional CT (4DCT) and cine-MRI in patients with hepatic tumors treated with radiation therapy. Methods and Materials: Patients with liver tumors who underwent 4DCT and 2-dimensional biplanar cine-MRI scans during simulation were retrospectively reviewed
to determine the extent of target motion in the superior-inferior, anteriore-posterior, and lateral directions. Cine-MRI was performed over 5 minutes. Tumor motion from MRI was determined by tracking the centroid of the gross tumor volume using deformable image registration. Motion estimates from 4DCT were performed by evaluation of the fiducial, residual contrast (or liver contour) positions in each CT phase. Results: Sixteen patients with hepatocellular carcinoma (n=11), cholangiocarcinoma (n=3), and liver metastasis (n=2) were reviewed. Cine-MRI motion was larger than 4DCT for the superior-inferior direction in 50% of patients by a median of 3.0 mm (range, 1.5-7 mm), the anterior-posterior direction in 44% of patients by a median of 2.5 mm (range, 1-5.5 mm), and laterally in 63% of patients by a median of 1.1 mm (range, 0.2-4.5 mm).