Restoration of
CXCL14 expression inhibited proliferation, migration and invasion in HCT116 cells. NF-κB signaling was suppressed when restoration of CXCL14 expression in HCT116 cells. Conclusion: CXCL14 is frequently methylated in human colorectal cancer and promoter region hypermethylation silenced CXCL14 expression in colorectal cancer cell lines. Alvelestat research buy Colorectal cancer cell proliferation, migration and invasion were inhibited by suppressing NF-κB signaling pathway. Key Word(s): 1. CXCL14; 2. colorectal cancer; 3. DNA methylation; Presenting Author: QIN NIAN Additional Authors: Corresponding Author: QIN NIAN Affiliations: RenMin Hospital of WuHan University Objective: To research the expression of PKM2 in adenocacinoma,
adenoma, inflammatory polyp and nomal mucous membrane of colon and to explore the clinical significance. To observe the inhibitory effects on the growth and the changes of PKM2 Selleck Saracatinib expression of colon cancer cells after treatment with ursolic acid and explore its mechanism of effect. Methods: Immunohistochemistry was used to detect the expression of PKM2 in adenocacin- oma, adenoma, inflammatory polyp and nomal mucous membrane of colon with 30 cases in each, taken by surgical or endoscopy operation and diagnosed definitely by pathology. The MTT assay was used to detect the cell growth rate with different concentrations of urolic acid (20 μmol/L, 40 μmol/L, 60 μmol/L, 80 μmol/L) after 24 h, 48 h, 72 h respectively. Western Blot was used to detect the changes of PKM2 expression in colon cancer cells after treatment with ursolic acid of 40 μmol/L, 60 μmol/L, 80 μmol/L. Results: The positive 上海皓元医药股份有限公司 rate of PKM2 expression was 0, 6.67%, 16.67% and 80.00% respectively in nomal mucous membrane, inflammatory
polyp, adenoma, adenocacinoma of colon. There were statistical differences (P < 0.001) between which in colorectal adenocarcinoma and nomal mucous membrane (χ2 = 40.00), inflammatory polyp (χ2 = 32.85), adenoma (χ2 = 24.09) of colon. PKM2 expression in colorectal adenocarcinoma was found to be correlated with lymph node metastasis (P = 0.046) and Dukes stage (P = 0.013), but not to be relative to the degree of tumor differentiation (P = 0.053/0.371) or the depth of invasion (P = 0.084). Ursolic acid can inhibit the growth of colon cancer cells Lovo in concentration-and time-dependent manners, and the IC50 after 24 h, 48, 72 h were 74.05 μmol/L, 57.75 μmol/L, 43.96 μmol/L respectively. Compare to the blank control group, PKM2 protein expression in the colon cancer cells Lovo decreased in concentration manners after treatment with ursolic acid of 40 μmol/L, 60 μmol/L, 80 μmol/L. Making β-actin as a reference protein, the protein expression values of PKM2 (%) were 0.8124 (the blank control group), 0.6671 (40 μmol/L ursolic acid group), 0.5268 (60 μmol/L ursolic acid group), 0.4081 (80 μmol/L ursolic acid group) respectively.