Fat content is the most variable component of milk; it is influenced by the lactation stage, breed and animal genotype, as well as by season and feed (Raynal-Ljutovac, Lagriffoul, Paccard, Guillet, & Chilliard, 2008). Lipolysis is the spontaneous enzymatic hydrolysis of fat, which in milk depends on physiological conditions, lactation period and animal genetic characteristics (Raynal-Ljutovac et al., 2005). The fatty acid contents of Coalho cheeses made from cow’s, goat’s milk and their mixture after 14 and 28 days of storage at 10 °C are shown in Table 2. The

total fatty acids content BTK pathway inhibitors found in the different cheeses showed no difference (P > 0.05) during storage. However, the individual content of C6, C8, C10, C12, C14, C16, C16:1 and C18:2n6c were

significantly different (P < 0.05) among the evaluated cheeses. CCGM and CGM showed higher (P < 0.05) contents of short- and of medium-chain fatty acids, such as C6 (caproic acid), C8 (caprylic acid) and C10 (capric acid). Higher amounts of C12 (lauric acid) in CGM were only found after 28 days of storage. Chilliard, Rouel, and Leroux (2006) state that milk from small ruminants presents high amounts of short- and medium-chain fatty acids, which are characteristically more pronounced in goat's milk. According to these authors, the amounts of fatty acids C6–C10 are at least two-fold higher in goat's Stem Cell Compound Library research buy milk than in cow’s milk. CCM presented higher amounts of C16 (palmitic acid) and C16:1 (palmitoleic acid) than CGM and CCGM after both evaluated storage times. These results are in accordance with those reported by Ceballos et al. (2009) and Lucas, Rock, Agabriel, Chilliard, and Coulon (2008), who reported higher contents of C6, C8,

C10 and C12 fatty acids in cheeses made from goat’s milk, while in cheeses made from cow’s milk, higher amounts of C14, C16, C16:1 and C20:3n6 were found. Delgado, González-Crespo, MYO10 Cava, and Ramírez (2011) found similar amounts of C6–C12 fatty acids in Iberian cheeses made from goat’s milk in Southwest Spain. The different quantitative profiles of fatty acids between CCM and CGM could be related to the different physiological regulation of mammary glands between the bovine and caprine species, particularly in the elongation process of fatty acids, which are synthesized by the fatty acids synthesis complex (Lucas et al., 2008). The highest amounts of C18:2n6c (linoleic acid) were found in CGM at both evaluated storage periods. CGM also presented higher amounts of C18:2n6c compared to CCM, suggesting that the inclusion of goat’s milk was responsible for the increase in the amount of this fatty acid. Chilliard et al. (2006) state that short- and medium-chain fatty acids only arise from synthesis in the mammary gland, while long-chain fatty acids (C ≥ 18) in milk fat originate from either dietary fat or body fat mobilization.