18 However, because MDR3 is activated by both the addition of bezafibrate as well as by UDCA monotherapy,7 the roles of bezafibrate in the combination therapy remain unknown. The current study was undertaken to explore the mechanisms of the remission of cholestasis by bezafibrate in PBC

patients who failed to respond to UDCA monotherapy. Our in vivo and in vitro studies demonstrated that bezafibrate was a dual PPARs/pregnane X receptor (PXR; NR1I2) agonist with potent anticholestatic efficacy. ABC, ATP-binding cassette transporter; BSEP, bile salt export pump; C4, 7α-hydroxy-4-cholesten-3-one; CA, cholic acid; CAR, constitutive androstane receptor; CDCA, chenodeoxycholic acid; DCA, BAY 73-4506 chemical structure deoxycholic acid; FGF, fibroblast growth factor; FXR, farnesoid X receptor; 4β-HC, 4β-hydroxycholesterol; 24S-HC, 24S-hydroxycholesterol; 27-HC, 27-hydroxycholesterol;

HMGCR, HMG-CoA reductase; HNF4α, hepatocyte nuclear factor 4α; LCA, selleck chemical lithocholic acid; LXRα, liver X receptor α; MDR, multidrug resistance protein; MRP, multidrug resistance-associated protein; NF-κB, nuclear factor-κB; NTCP, Na+/taurocholate cotransporting polypeptide; PBC, primary biliary cirrhosis; PPAR, peroxisome proliferator-activated receptor; PXR, pregnane X receptor; PGC1α, peroxisome proliferator-activated receptor-γ coactivator-1α; UDCA, ursodeoxycholic acid. Thirty-one Japanese patients with asymptomatic and untreated PBC (4 males and 27 females; ages 37-81 this website years) were enrolled in the

study. The diagnosis of PBC was established by laboratory and histological findings, and all patients were classified as early-stage PBC (Scheuer’s classification I or II). Informed consent was obtained from all subjects and the study protocol was approved by the Ethics Committee of Tokyo Medical University Ibaraki Medical Center. All patients (n = 31) were treated with UDCA (600 mg/day; 10-13 mg/kg/day) alone for at least 3 months (maximum 6 months) until serum ALP and gamma glutamyl transpeptidase (GGT) became stable (Supporting Figure). Then bezafibrate (400 mg/day) was administered with UDCA (600 mg/day) to patients (n = 19; 1 male and 18 females) who exhibited an incomplete biochemical response to UDCA monotherapy (defined as ALP or GGT level of above the upper limit of normal) and treated for 3 months. Before and after UDCA monotherapy and after the addition of bezafibrate, blood samples were collected in the morning before breakfast after an overnight fasting, and serum was stored at −20°C until analyzed. Control sera from 49 healthy Japanese volunteers (11 males and 38 females; ages 22-79 years) were obtained from another study group (courtesy of Prof. T.