This could indicate a problem with compliance. However, participants took 100,000 IU under supervision, and exactly the same pattern is observed in the 800 IU group and the sunlight group. This may indicate that supplementation was inadequate. A dose-finding study in nursing home residents Raf inhibitor studied with the same 25(OH)D assays showed that serum 25(OH)D was higher than

50 nmol/l with vitamin D 600 IU/day in 90% of the participants [33]. This fact and the decrease in serum 25(OH)D between 3 and 6 months (Fig. 2, Table 2) indicate a compliance problem. Another point of concern is the interaction of the increase of serum 25(OH)D after supplementation with BMI, mainly in the 100,000 IU group. Although this analysis should be considered exploratory, Tozasertib solubility dmso it may indicate that overweight and obese persons will need higher supplementation doses. The negative relationship between body fat percentage and serum 25(OH)D has been reported in the Longitudinal Aging Study Amsterdam [34]. It is striking that PTH concentrations decreased most in the100,000 IU group, although serum 25(OH)D concentrations increased most in the 800 IU group. This might be due to a higher peak concentration of serum PTH in the 100,000 IU group. The mean serum alkaline EPZ015938 phosphatase decreased in all groups by about 20%. The high

alkaline phosphatase is a sign of high bone turnover or disturbed mineralization due to vitamin medroxyprogesterone D deficiency. Besides serum 25(OH)D and PTH concentrations, several clinical outcomes were studied. An improvement in physical performance was not observed. Difficulties with daily life activities decreased significantly, but no differences were observed between the interventions. This may indicate that only a small improvement in vitamin D status is needed to improve functional limitations. Reported pain was not consistent over time or between interventions: number of days with headache episodes decreased

significantly among participants in the 800-IU intervention and reported pain in upper legs improved significantly in the 100,000-IU intervention compared to the advised sunlight intervention, but no improvement was observed in shoulder pain. The inconsistent clinical results can be explained by the methodological restrictions of this study. There was no placebo-control group as this was judged unethical in this vitamin D-deficient population. Handgrip strength is known to be positively correlated with both lower-extremity and upper-body strength, and it appears to be a reliable test [35, 36]. The chair stand test is reliable and related to vitamin D status [14], but both relationships have been established in older populations. The impact of vitamin D deficiency on muscle strength could be less in younger persons than in older persons. In addition, the tests could not be sufficiently discriminative in a younger population.