Results: The extract exhibited good antibacterial activity against all of the clinical isolates of S. pyogenes with similar MIC and MBC ranging from 3.91-62.50 mu g/ml. Complete killing of the bacterial
cells by the extract at concentrations of 4MIC, 2MIC, and MIC occurred within selleck 4, 8, and 12 h, respectively. Treatment of the bacterial cells with the extract at 2MIC and 4MIC caused cell lysis. All the test concentrations (1/32 – 1/2MIC) produced no effects on protease and haemolysin enzymes.
Conclusion: Boesenbergia pandurata extract has powerful in vitro activity against S. pyogenes. The ability of the extract to lyse the bacterial cells suggests that the mechanism of action may be associated with cell wall and cell membrane damage.”
“Background and objective: Interstitial lung diseases (ILD) are characterized by progressive interstitial pulmonary fibrosis and a decline
in lung function. Fibrocytes are bone marrow-derived mesenchymal progenitor FK228 in vitro cells that may play a role in the pathogenesis of pulmonary fibrosis. Circulating fibrocyte numbers have been correlated with the prognosis of patients with idiopathic pulmonary fibrosis. The aim of the present study was to evaluate the relationship between circulating fibrocytes, and parameters of disease activity and progression in several groups of patients with ILD. Methods: The study population comprised 41 patients with ILD and seven healthy control subjects. Circulating CD45+ collagen-I+ fibrocytes were evaluated by flow cytometry. Results: The number of circulating fibrocytes was significantly increased in all patients with ILD and particularly in patients with idiopathic interstitial pneumonitis and
interstitial pneumonitis associated with collagen vascular disease as compared with healthy control subjects. The numbers CH5183284 in vivo of circulating fibrocytes were significantly correlated with pulmonary function test parameters and with serum levels of sialylated carbohydrate antigen, a marker of disease activity. Temporal changes in circulating fibrocyte numbers were evaluated in two patients, and the results suggested that these changes correlated with the activity of ILD. Conclusions: The results from this study provide further evidence for the role of circulating fibrocytes in fibrotic lung diseases.”
“Purpose: The expression of FasR, Fas-L and Bcl-2 on CD4(+) and CD8(+) T lymphocytes subpopulations from the cord blood were assayed. The results in blood from boys and girls were analyses separately and compared.
Material and Methods: Twenty four full-term newborns: 13 females and 11 males were included into the study. Blood from the umbilical vein was collected immediately after cutting the umbilical cord. The staining with monoclonal antibodies against CD4, CD8, FasR, Fas-L and Bcl-2 was performed within 2 hours after collection and followed with flow cytometry acquisition and analysis.