Furthermore, we found that intrahepatic IL-17 was mainly localized in the fibrosis region. Our data reveal important roles of IL-17 and IL-17-producing cells in the progression of HBV related chronic liver diseases, especially in the formation of liver fibrosis.”
“Aims: In a previous study we found that A-935142 enhanced hERG current in a concentration-dependent manner by

facilitating activation, Small molecule library cell line reducing inactivation, and slowing deactivation (Su et al., 2009). A-935142 also shortened action potential duration (APD(90)) in canine Purkinje fibers and guinea pig atrial tissue. This study focused on the combined effects of the prototypical hERG enhancer, A-935142 and two hERG current blockers (sotalol and terfenadine).\n\nMain methods: The whole-cell voltage clamp method with HEK 293 cells heterologously expressing the hERG channel (Kv 11.1) was used.\n\nKey findings: A-935142 did not compete with H-3-dofetilide binding, suggesting that A-935142 does not overlap the binding site of typical hERG blockers. In whole-cell voltage clamp studies we found: GSK923295 1) 60 mu M A-935142 enhanced hERG current in the

presence of 150 mu M sotalol (57.5 +/- 5.8%) to a similar extent as seen with A-935142 alone (55.6 +/- 5.1%); 2) 150 mu M sotalol blocked hERG current in the presence of 60 mu M A-935142 (43.5 +/- 1.5%) to a similar extent as that seen with sotalol alone (42.0 +/- 3.2%) and 3) during co-application, hERG current see more enhancement was followed by current blockade. Similar results were obtained with 60 nM terfenadine combined with A-935142.\n\nSignificance: These results suggest that the hERG enhancer, A-935142 does not compete with these two known hERG blockers at their binding site within the hERG channel. This selective hERG current enhancement may be useful as a treatment for inherited

or acquired LQTS (Casis et al., 2006). (c) 2012 Elsevier Inc. All rights reserved.”
“Background: Globally, BCG vaccination varies in efficacy and has some non-specific protective effects. Previous studies comparing BCG strains have been small-scale, with few or no immunological outcomes and have compared TB-specific responses only. We aimed to evaluate both specific and non-specific immune responses to different strains of BCG within a large infant cohort and to evaluate further the relationship between BCG strain, scarring and cytokine responses.\n\nMethods: Infants from the Entebbe Mother and Baby Study (ISRCTN32849447) who received BCG-Russia, BCG-Bulgaria or BCG-Denmark at birth, were analysed by BCG strain group. At one year, interferon-gamma (IFN-gamma), interleukin (IL)-5, IL-13 and IL-10 responses to mycobacteria-specific antigens (crude culture filtrate proteins and antigen 85) and non-mycobacterial stimuli (tetanus toxoid and phytohaemagglutinin) were measured using ELISA.