ACME Laboratories is ISO 9001 certified. Four method blanks were analyzed during this study and several elements were detected at concentrations just above detection limits in one of the four method blanks. They included Ba (0.07 ppb), Be (0.06 ppb), Ru (0.07 ppb), S (1 ppm), and Sr (0.05 ppb). Four pairs of duplicate samples were analyzed and the average relative percentage difference Selleck Buparlisib (RPD)

for Al, Ca, and K was <1%. For Ba, Cl, Na, Nd, Rb, Si, and Sr the RPD varied between 1–5%. For Cr, Ce, La, Li, and Zn the RPD varied between 5 and 10%. Elements with higher average RPD include B (13.3%), Cu (22.2%), Fe (14.3%), Ni (14.3%), S (22.2%), Y (35.6%), and Zr (28.6%). In general, the RPD between duplicate samples of each element was inversely proportional to their overall concentration. Repeat analysis of a certified lake water standard (TMDA-70) indicated major components of the water were accurate well within 20% with CDK inhibitor one exception Si (23.3%). Prior to ion chromatography analysis certified reference standards were run. Standards included Fluka multi-anion standard (89886-50 mL-F) for F, Cl, Br, NO3, PO4, SO4, Fluka (72784-1 L-F) for CO3, and Fluka (36427-100 ml-F) for NO2. If the values determined for the reference

standard differed from the accepted value by more than 5% for each analyte the instrument was recalibrated until this limit was achieved. The method detection limits were calculated by performing seven replicate injections of nanopure water fortified at a concentration of three to five times the estimated instrument detection limits then adjusted Buspirone HCl downwards. Duplicate samples indicate reported values for each anion had a RPD of 10% or less. None of the anions were found above detection limits in blank samples. Recovery of standards based on seven injections ranged from 95.1 (CO3) to 106% (NO2-N). The data were compiled and summarized in Excel® spreadsheets. Standard statistical parameters (mean,

standard deviation, relative percent difference, etc.) were determined through the use of an Excel spreadsheet and used to determine the quality and range of the data and display relevant results. Correlation coefficients (r2) were calculated to determine the potential correlation between various analytes and other parameters and concentration trends with distance downriver. During the September 4th, 2011 Tropical Storm Irene stormflow sampling event the pH of water in the Raquette River ranged from 5.20 to 6.47, with the exception of a single sample collected at Raymondville which had a pH of 8.21 (Supplemental Table 3). Because this was clearly an outlier, the pH was measured several times for verification with the same result. The specific conductance during this sampling event ranged from 22.98 to 65.06 μS cm−1, with the sole exception of the Raymondville sample which was anomalous at 160.44 μS cm−1. Water temperature ranged from 21.2 to 25.